Proteomics Sample Preparation Guidelines

Cells and Tissue Samples

Special Tips for Sample Handling

Please ship pellets (cells) or tissue lysate solutions on dry-ice (avoid freeze/traw cycles) via overnight courier together with a filled Omics Technologies Proteomics Sample Submission Form.

Please follow the following protocol for cell collecting or cell lysate collecting:

1, Remove culture media as complete as possible;

2, Wash the cells with chilled (4°C) PBS for 2 times, each with 1/2 volume of the culture media you used;

3, Wash the cells with chilled (4°C) PBS containing protease inhibitors for 1 time, with 1/2 volume of the culture media you used;

4, Scrape the cells and collect them at a cornor of your plate/flask, and pipette the cells up and down multiple times;

5, Transfer the cells (respsuended in PBS with protease inhibitors) to a 10ml tube or 1.5ml tube;

6, Spin the tube at 1000rpm for 1 min at 4°C;

7, Remove the supernatant as complete as possible;

8, a) For sending cell pellet to us: Freeze the cell pellet with liguid nitrogen and keep them in your -80°C freezer. Please avoid putting them in -20°C freezers;

8, b) For sending cell lysate to us: Use your favorite lysis buffer to extract the total protein lysates and then freeze the cell lysates with liguid nitrogen and keep them in your -80°C freezer. Please avoid putting them in -20°C freezers;

9, Send the cell pellets/lysates to us on dry ice.

Please avoid using Trypsin during cell pellet/lysate collecting. Instead, please physically scrape them off the plate or flask using mechanical forces.

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